The WIOpi-01 PCB expects the Zero header at the bottom side:

Buy a Zero type W, and not type WH. The header of type WH is on top.
 
If a Zero with the header on top is already available, the Zero connector on the WIOpi-01 can also be mounted at the bottom side of the PCB.
 
The WS2801 LED strips connection:
| Pixel | I/O1 | BCM | Remark | 
|---|---|---|---|
| GND | 2 (GND) | - | |
| DI | 6 (spi0) | MOSI | |
| CI | 8 (spi2) | SCLK | |
| V+ | 1 (VDD) | - | Only to feed a few WS2801. | 
 
| Stepper | I/O2 | BCM | Remark | 
|---|---|---|---|
| GND | 2 (GND) | - | |
| IN1 | 3 (st1-0) | 0 | |
| IN2 | 4 (st1-1) | 5 | |
| IN3 | 5 (st1-2) | 6 | |
| IN4 | 6 (st1-3) | 19 | |
| CAL | 8 (st1-5) | 26 | |
| V+ | 1 (VDD) | - | Only to feed a small 5V stepper. |